Publications
2014
1. Goessweiner-Mohr, N., Eder, M., Hofer, G., Fercher, G., Arends, K., Birner-Gruenberger, R., Grohmann, E., and Keller, W. (2014). Structure of the double stranded DNA binding type IV secretion protein TraN from Enterococcus. Acta Crystallographica Section D (in press).
2. Broszat, M., Nacke, H., Blasi, R., Siebe, C., Hübner, J., Daniel, R., Grohmann, E. (2014). Wastewater irrigation increases abundance of potentially harmful Gammaproteobacteria in soils from Mezquital Valley, Mexico. Appl. Environ. Microbiol. (in press).
3. Yamaguchi, N., Roberts, M., Castro, S., Oubre, C., Makimura, K., Leys, N., Grohmann, E., Sugita, T., Ichijo, T., and Nasu M. (2014). Microbial monitoring of crewed habitats in space - current status and future perspectives. Microbes and Environments (Invited Mini-Review) (in press).
4. Goessweiner-Mohr, N., Arends, K., Keller, W., and Grohmann, E. (2014). Conjugation in Gram-positive bacteria. Microbiol. Spectrum 2(3): PLAS-0004-2013. doi:10.1128/microbiolspec.PLAS-0004-2013. (Invited Review) (in press)
5. Goessweiner-Mohr, N., Fercher, C., Arends, K., Birner-Gruenberger, D., Laverde-Gomez, D., Huebner, J., Grohmann, E., and Keller, W. The type IV secretion protein TraK from the Enterococcus conjugative plasmid pIP501 exhibits a novel fold. (2014). Acta Crystallogr Sect D 70(Pt 4):1124-1135.
6. Broszat, M., Grohmann, E. (2014). Book chapter and co-editor: Spread of antibiotic resistance in the environment: Impact on human health. In Environmental Deterioration and Human Health: Natural and Anthropogenic Determinants. Abdul Malik, Elisabeth Grohmann, Rais Akhtar Editors. Springer press, New York. ISBN 978-94-007-7890-0
7. Broszat, M., Grohmann, E. (2014). Book chapter: Horizontal gene transfer
in biofilm and planktonic modes. In Antibiofilm agents: From
Diagnosis to Treatment and Prevention, Series: ?Springer Series on Biofilms?, Vol. 8. Iqbal Ahmad, Kendra Rumbaugh Editors. Springer press, New York. ISBN 978-3-642-53833-9
2013
Arends, K., Celik, E.-K., Probst, I., Goessweiner-Mohr, N., Fercher, C., Grumet, L., Soellue, C., Abajy, M.Y., Sakinc, T., Broszat, M., Schiwon, K., Koraimann, G., Keller, W. and Grohmann, E. TraG encoded by the pIP501 type IV secretion system is a two domain peptidoglycan degrading enzyme essential for conjugative transfer. (2013). J. Bacteriol. 195 (19): 4436-4444.
Goessweiner-Mohr, N., Arends, K., Keller, W., and Grohmann, E. Conjugative Type IV Secretion in Gram-positive bacteria. (2013). Plasmid (Invited Mini-Review) 70 (3): 289-302.
Werner, G., Coque, T. N., Franz, C. M. A. P., Grohmann, E., Hegstad, K., Jensen, L., Sundsfjord, A., van Schaik, W., Weaver, K., and Willems, R.J. (2013). Antibiotic resistant Enterococci – tales of a resistance gene trafficker. Invited Review for Int. J. Med. Microbiol. 303:360-379.
Gössweiner-Mohr, N., Grumet, L., Arends, K., Pavkov-Keller, T., Gruber, C., Birner-Gruenberger, R., Kropec-Huebner, A., Huebner, J., Grohmann E., and Keller, W. (2013). The 2.5 Å structure of the Enterococcus conjugation protein TraM resembles VirB8 type IV secretion proteins. J. Biol. Chem. 288(3):2018-2028.
Gössweiner-Mohr, N., Grumet, L., Pavkov-Keller, T., Birner-Gruenberger, R., Grohmann, E., and Keller, W. (2013). Crystallization and preliminary structure determination of the transfer protein TraM from the Gram-positive conjugative plasmid pIP501. Acta Crystallographica Struct Biol Cryst Commun. Section F 69(Pt 2):178-183.
Schiwon, K., Arends, K., Preschan, K., Sakinc, T., Hahn, S., van Houdt, R., Werner, G., and Grohmann, E. (2013). Comparison of antibiotic resistance, biofilm formation and conjugative transfer of Staphylococcus and Enterococcus isolates from International Space Station (ISS) and Antarctic base Concordia. Microb. Ecol. 65(3):638-651.
Grohmann, E. (2013). Book chapter and co-editor: Conjugative plasmids in anthropogenic soils. In Management of Microbial Resources in the Environment. Abdul Malik, Elisabeth Grohmann, Madalena Alves Editors. ISBN 978-94-007-5931-2. Springer press, New York.
2012
Gössweiner-Mohr, N., Fercher, C., Abajy, M.Y., Grohmann, E., and Keller, W. (2012). Crystallization and preliminary structure determination of the putative transfer protein TraN from Gram-positive conjugative plasmid pIP501. Acta Crystallographica Struct Biol Cryst Commun. Section F 68(Pt 11):1402-1405.
Arends, K., Schiwon, K., Sakinc, T., Huebner, J., and Grohmann, E. (2012). A GFP-labeled monitoring tool to quantify conjugative plasmid transfer between Gram-positive and Gram-negative bacteria. Appl. Environ. Microbiol. 78(3):895-899.
Anjum, R., Grohmann, E., and Malik A. (2012). Exogenous isolation of conjugative plasmids from pesticide contaminated soil. World J. Microbiol. Biotechnol. 28(2):567-574.
Levantesi, C., Briancesco, F., Bonadonna, L., Grohmann, E., Toze, S., and Tandoi, V. (2012). Salmonella in surface and drinking water: occurrence and waterborne transmission. Food Research International. Invited Review Special Issue 45(2): 587-602.
V. Tandoi, C. Levantesi, S. Toze, U. Böckelmann, M. Divizia, N. Ayuso, M. Salgot, R. La Mantia, E. Grohmann. (2012). Book chapter: Water quality analysis – microbiological hazards. In Advances in Water Reclamation Technologies for Safe Managed Aquifer Recharge: Christian Kazner, Thomas Wintgens, Peter Dillon Editors. ISBN: 9781843393443. IWA Publishing, London, UK.
2011
Kropec, A., Sava, I. G., Vonend, C., Sakinc, T., Grohmann, E., and Huebner, J. (2011). Identification of SagA as a novel vaccine target for the prevention of Enterococcus faecium infections. Microbiology 157(12):3429-3434.
Theilacker, C., Sava, I., Sanchez-Carballo, P., Bao, Y., Kropec, A., Grohmann, E., Holst, O., and Huebner, J. (2011). Deletion of the glycosyltransferase bgsB of Enterococcus faecalis leads to a complete loss of glycolipids from the cell membrane and to impaired biofilm formation. BMC Microbiology 11(1):67.
Anjum, R., Grohmann, E., and Malik, A. (2011). Molecular characterization of conjugative plasmids in pesticide tolerant and multi-resistant bacterial isolates from contaminated alluvial soil. Chemosphere 84(1):175-181.
Grohmann, E. (2011). Book chapter: Horizontal gene transfer between bacteria under natural conditions. In Microbes and Microbial Technology: Agricultural and Environmental Applications: Iqbal Ahmad Farah Ahmad, John Pichtel Editors. ISBN 978-1-4419-7930-8, e-ISBN 978-1-4419-7931-5. DOI 10.1007/978-1-4419-7931-5. Springer New York Dordrecht Heidelberg London© Springer Science+Business Media, LLC 2011.
Grohmann, E., and Arends, K. Book chapter and co-editor: Molecular detection of resistance and transfer genes in environmental samples. (2011). In Environmental Protection Strategies for Sustainable Development. Abdul Malik and Elisabeth Grohmann (eds.), Springer press, New York, ISBN: 978-94-007-1590-5. http://www.springer.com/environment/sustainable+development/book/978-94-007-1590-5.
Ansari, M. I., Schiwon, K., Malik, A., and Grohmann, E. Book chapter and co-editor: Biofilm formation by environmental bacteria. (2011). In Environmental Protection Strategies for Sustainable Development. Abdul Malik and Elisabeth Grohmann (eds.), Springer press, New York, ISBN: 978-94-007-1590-5. http://www.springer.com/environment/sustainable+development/book/978-94-007-1590-5.
Malik, A., Rahman, M., Ansari, M. I., Masood, F., and Grohmann, E. Book chapter and co-editor: Environmental Protection Strategies: An Overview. (2011). In Environmental Protection Strategies for Sustainable Development. Abdul Malik and Elisabeth Grohmann (eds.), Springer press, New York, ISBN: 978-94-007-1590-5. http://www.springer.com/environment/sustainable+development/book/978-94-0071590-5.
2010
Levantesi, C., La Mantia, R., Masciopinto, C., Böckelmann, U., Ayuso-Gabella, M. N., Salgot, M., Tandoi, V., Van Houtte, E., Wintgens, T., and Grohmann E. (2010). Quantification of pathogenic microorganisms and microbial indicators in three wastewater reclamation and managed aquifer recharge facilities in Europe. Sci. Total Environ. 408(21):4923-4930.
Braun, B., Böckelmann, U., Grohmann, E., and Szewzyk, U. (2010). Bacterial soil communities affected by water repellency. Geoderma. 158(3-4):343-351. doi:10.1016/j.geoderma.2010.06.001
Rangrez, A.Y., Abajy, M.Y., Keller, W., Shouche, Y., and Grohmann, E. (2010). Biochemical characterization of three putative ATPases from a new type IV secretion system of Aeromonas culicicula plasmid pAC3249A. BMC Biochemistry 11:10.
Grohmann, E. (2010). Conjugative transfer of the integrative and conjugative element ICEBs1 from B. subtilis likely initiates at the donor cell pole. Guest commentary. J. Bacteriol. 192 (1):23-25.
Grohmann, E. (2010). Autonomous plasmid-like replication of Bacillus ICEBs1: a general feature of integrative conjugative elements? Micro Commentary Mol. Microbiol. 75(2):261-263.
2009
Borgo, F., Ricci, G., Arends, K., Schiwon, K., Grohmann, E., and fortina M.G. (2009). Evaluation of plasmid content and tetracycline resistance conjugative transfer in Enterococcus italicus strains of dairy origin. Curr. Microbiol. 59 (3):261-266.
Böckelmann, U., Dörries, H.-H., Ayuso-Gabella, M. N., Salgot de Marçay, M., Tandoi, V., Levantesi, C., Masciopinto, C., Van Houtte, E., Szewzyk, U., Wintgens, T., and Grohmann, E. (2009). Quantitative real-time PCR monitoring of bacterial pathogens and antibiotic resistance genes in three European artificial groundwater recharge systems. Appl. Environ. Microbiol. 75 (1): 154-163.
2008
Ansari, M. I., Grohmann, E., and Malik, A. (2008). Conjugative plasmids in multi-resistant bacterial isolates from Indian soil. J. Appl. Microbiol. 104(6):1774-1781.
Fortina, M. G., Ricci, G., Borgo, F., Manachini, P. L., Arends, K., Schiwon, K., Abajy, M. Y., and Grohmann, E. (2008). A survey on biotechnological potential and safety of the novel Enterococcus species of dairy origin, E. italicus. Int. J. Food Microbiol. 123 (3): 204-211.
Malik, A., Celik, E.-K., Bohn, C., Boeckelmann, U., Knobel, K., and Grohmann, E. (2008). Molecular detection of conjugative plasmids and antibiotic resistance genes in anthropogenic soils from Germany and India. FEMS Microbiol. Lett. 279 (2): 207-216.
Böckelmann, U., Dörries, H.-H., Szewzyk, U., and Grohmann, E. Quantitative PCR monitoring of bacterial pathogens and antibiotic resistance genes in the artificial groundwater recharge systems Torreele, Sabadell and Nardo. Final Dissemination Workshop on Water Reclamation and Aquifer Recharge, 5.-6.9.2008, Maribor, Slovenia. In Water Reclamation and Aquifer Recharge, IEI (Institute for Ecological Engineering), Maribor, Slovenia, pp. 48-49. ISBN: 978-961-238-954-3.
2007
Abajy, M. Y., Kopec, J., Schiwon, K., Burzynski, M., Döring, M., Bohn, C., and Grohmann, E. (2007). A type IV-secretion-like system (T4SLS) is required for conjugative DNA transport of plasmid pIP501 with broad host range in Gram positive bacteria J. Bacteriol. 189: 2487-2496.
Schaumann, G. E., Braun, B., Kirchner, D., Rotard, W., Szewzyk, U., and Grohmann, E. (2007). Influence of biofilms on the water repellency of urban soil samples. Hydrological Processes (special issue on water repellency) 21 (17): 2276-2284, online ISSN: 1099-1085, print ISSN: 0885-6087.
Alexandrino, M., Grohmann, E., Szewzyk, R., and Szewzyk, U. (2007). Application of culture-independent methods to assess the microbial purification efficiency of subsurface flow constructed wetlands. Water Sci. Technol. 56 (3): 217-222.
2006
Kurenbach, B., Kopeć, J., Mägdefrau, M, Andreas, K., Keller, W., Bohn, C., Abajy, M. Y., and Grohmann, E. (2006). The TraA relaxase autoregulates the putative type IV secretion-like system encoded by the broad-host-range Streptococcus agalactiae plasmid pIP501. Microbiology 152 (3): 637-645.
Braun, B., Böckelmann, U., Grohmann, E., and Szewzyk, U. (2006). Polyphasic characterization of the bacterial community in an urban soil profile with in situ and culture dependent methods. Appl. Soil. Ecol. 31 (3): 267-279.
Jäger, F., Grohmann, E., and Schaumann, G. E. (2006). H NMR relaxometry in natural humous soil samples: Insights in microbial effects on relaxation time distributions. Plant and Soil 280 (1-2): 209-222. DOI 10.1007/s11104-005-3035-4.
Grohmann, E. (2006). Book chapter: Mating cell-cell channels in conjugating bacteria. In Cell-Cell Channels (eds. Baluska, F., Volkmann, D., and Barlow, P. W.), Landes Biosciences, Georgetown, Texas, pp. 21-38. ISBN: 1-58706-065-5. http://eurekah.com/abstract.php?chapid=2730&bookid=192&catid=16.
2005
Kopeć, J., Bergmann, A., Fritz, G., Grohmann, E., and Keller, W. (2005). TraA and its N-terminal relaxase domain of the Gram-positive plasmid pIP501 show specific oriT binding and behave as dimers in solution. Biochem. Journal 387 (2): 401-409.
2004
Alexandrino, M., Grohmann, E., and Szewzyk, U. (2004). Optimization of PCR based methods for rapid detection of pathogens in wastewater samples. Wat. Res. 38 (5): 1340-1346.
Grohmann, E. Plasmidtransfer in Gram-positiven Bakterien: ein neuer Typ IV-Sekretionsmechanismus? BIOspektrum 3/2004, p. 282.
2003
Kurenbach, B., Bohn, C., Prabhu, J., Abudukerim, M., Szewzyk, U., and Grohmann, E. (2003). Intergeneric transfer of the Enterococcus faecalis plasmid pIP501 to Escherichia coli and Streptomyces lividans and sequence analysis of its tra region. Plasmid 50: 86-93.
Böckelmann, U., Szewzyk, U., and Grohmann, E. (2003). A new enzymatic method for the detachment of particle associated soil bacteria. J. Microbiol. Methods 55 (1): 201-211.
Grohmann, E., Muth, G., and Espinosa, M. (2003). Conjugative plasmid transfer in gram-positive bacteria. Microbiol. Mol. Biol. Rev. 67 (2): 277-301.
2002
Kurenbach, B., Grothe, D., Farías, M. E., Szewzyk, U., and Grohmann, E. (2002). The tra-region of the conjugative plasmid pIP501 is organized in an operon with the first gene encoding the relaxase. J. Bacteriol. 184: 1801-1805.
2001
Farías, M. E., Ruiz-Maso, J. A., Grohmann, E., Guzmán, L., and Espinosa, M. Mechanisms of DNA-bound proteins in prokaryotes. Plasmid pMV158 encodes two proteins with nicking-closing activities: RepB, the initiator of replication, and MobM, involved in mobilisation. (2001). In Fundación Juan March (ed.), Mechanisms of DNA-bound proteins in prokaryotes, Fundación Juan March, Madrid, Spain, p. 63-64.
2000
Smalla, K., Krogerrecklenfort, E., Heuer, H., Dejonghe, W., Top, E., Osborn, M., Niewint, J., Tebbe, C., Barr, M., Bailey, M., Greated, A., Thomas, C., Turner, S., Young, P., Nikolakopoulou, D., Karagouni, A., Wolters, A., van Elsas, J. D., Dronen, K., Sandaa, R., Borin, S., Prabhu, J., Grohmann, E., and Sobecky, P. (2000). PCR-based detection of mobile genetic elements in total community DNA. Microbiology 146: 1256-1257.
1999
Grohmann, E., Guzmán, L., and Espinosa, M. (1999). Mobilisation of the streptococcal pMV158: interactions of MobM protein with its cognate oriT DNA region. Mol. Gen. Genet. 261: 707-715.
Farías, M. E., Grohmann, E., and Espinosa, M. (1999). Expression of the mobM gene of the streptococcal plasmid pMV158 in Lactococcus lactis subsp. lactis. FEMS Microbiol. Lett. 176: 403-410.
1998
Grohmann, E., Moscoso, M., Zechner, E. L., del Solar, G., and Espinosa, M. (1998). In vivo definition of the functional origin of leading strand replication on the lactococcal plasmid pFX2. Mol. Gen. Genet. 260: 38-47.
1997
Zechner, E. L., Prueger, H., Grohmann, E., Espinosa, M., and Hoegenauer, G. (1997). Specific cleavage of chromosomal and plasmid DNA strands in Gram-positive and Gram-negative bacteria can be detected in vitro with nucleotide resolution. Proc. Natl. Acad. Sci. USA. 94: 7435-7440.
Grohmann, E., Zechner, E. L., and Espinosa, M. (1997). Determination of specific DNA strand discontinuities with nucleotide resolution in exponentially growing bacteria harbouring rolling circle-replicating plasmids. FEMS Microbiol. Lett. 152: 363-369.
Grohmann, E., Stanzer, T., and Schwab, H. (1997). The ParB nuclease encoded by the RP4 par region is a Ca2+ dependent nuclease linearizing circular DNA substrates. Microbiology 143: 3889-3898.
1995
Jensen, R. B., Grohmann, E., Schwab, H., Diaz-Orejas, R., and Gerdes, K. (1995). Comparison of ccd of F, parDE of RP4, and parD of R1 using a novel conditional replication control system of plasmid R1. Mol. Microbiol. 17: 211-220.
1994
Eberl, L., Kristensen, C., Givskov, M., Grohmann, E., Gerlitz, M., and Schwab, H. (1994). Analysis of the multimer resolution system encoded by the parCBA operon of broad-host-range plasmid RP4. Mol. Microbiol. 12: 131-142.
1993
Haigermoser, C., Chen, G. Q., Grohmann, E., Hrabak, O., and Schwab, H. (1993). Stabilization of plasmid vectors by the partitioning function of broad-host-range plasmid RP4. J. Biotechnol. 28: 291-299.